许多糖尿病和肥胖研究研究依靠饮食诱导肥胖症（DIO）模型，但有许多选择和考虑因素，最大限度地提高了您的学习效果。两个EnB0B体育平台下载vigo科学家最近分享了他们关于如何选择和开发满足您研究需求的最佳DIO模型的见解。
1.评估您的学习设计和终点在比较饮食诱导的肥胖模型之前，重要的是考虑您的研究的假设，其设计和研究的终点。
“你研究糖尿病除了肥胖吗?Is your compound examining weight loss or the impedance of weight gain? These are important factors to communicate to your animal model vendor,” explained Mandy Horn, Scientist, Veterinary Science, Research & Support at Envigo. “Your vendor can help evaluate your study, learn when you want to induce DIO during the study, and use this information to determine which particular stock, strain or substrain would work best, as well as the help you determine how the sex of the mice may influence phenotype.”
2. Research historical control data and peer-reviewed publications
Published references can provide a wealth of information for spontaneous and diet induced metabolic models. Depending on the stock, strain and sex, models have varying responses to high fat diets. Envigo has publishedC57BL/6Hsd “Black 6” publications related to DIO并且可以提供RCCHAN的数据：巫师女性DIO。
“We’ve done in-house studies to optimize development of gonadal intact female Wistar Han rats to compare a variety of high fat diets and determine if they could create a reliable obesity model,” said Sheryl Wildt, Global Manager of Genetic Quality and Breeding.“我们发现了WistarHan®.female rats可以是测试新的抗肥胖化合物可以确定是否可以防止或减少肥胖，而不会产生以前健康的模特，培养不健康的副作用。“
“历史控制数据有助于为您的模型做出明智的决定，”曼迪补充道。“我们建议您评估模型的典型体重增加随着时间的推移，随着时间的推移可能会减慢或高原，而其他模型可能会继续井进入20-24周龄。”
3. Understand the differences with diet
Your animal model vendor will want to discuss the options for your models’ diet as it impacts the phenotype of the model. Diet vendors, including Teklad, can make any customized high-fat diet to meet the needs of your study. These vendors can alter the fat source or even adjust the ratio of omega-3 to omega-6 fatty acids or the ratio of long-chain to medium-chain fatty acids.
“有许多考虑因素，例如基于植物的脂肪，如椰子油或基于动物的脂肪，例如猪油，为您的脂肪来源，”曼迪解释道。“Diets can also have increased sugars, although with a higher fat content at 55-60%, the carbohydrates are relatively low when compared to other obesity inducing diets with 40-45% of the calories from fat, often referred to as the ‘Western Diet.’”
4.为成功进行学习
Once you have selected the ideal model for your study, you need to consider the age at which to start your models’ diet, based on the goals of your research. Typically, the high fat diet begins in mouse models around three to six weeks of age, depending on your study design and whether you are trying to treat or prevent obesity.
就住单小鼠而言，壳体密度会影响整体体重增加。共同饲养的小鼠可以比单胞埋的小鼠更快地体重，但这种差异可能在模型之间变化。对于丰富，嵌套材料，推荐用于DIO小鼠的嵌套纸和木材寝具。小鼠和大鼠有时会吃玉米衫床上用品，这可能对新陈代谢产生影响，为您的学习增加一个混杂。如果您的型号变得糖尿病，因此可能需要更频繁地改变床上用品，因为尿量增加。
与对照相比，在喂食一到两周后，可以注意到您的DIO组的重量增加，具体取决于该模型。某些型号可能不会显示六到八周的控制体重差异。“谢丽尔说。“其他更严重的表型可能需要超过10-12周才能出现。根据模型，体重增加可以持续到20周龄，研究人员应考虑到他们的学习的终点。“
5.外包您的DIO模型开发
创建自己的DIO模型的一个替代方案正在将工作外包给经过验证的供应商。B0B体育平台下载Envigo可以管理模型并开发它们以达到一定的重量或表型。
“典型时间轴需要6-8周;其他模型可能需要更多的时间，“曼迪解释道。“我们可以使用自己的Teklad饮食或与客户指定的饮食合作。我们管理库存并监控重量增益以提供学习准备的DIO型号。该过程是完全可定制的，以满足客户的研究需求。“
是否开发自己的DIO模型或外包，了解更多信息饮食在Envigo诱导肥胖症B0B体育平台下载。