Mice need time to adjust

计划：

• 食物摄入和减肥初步减少
• Daily observation of animal tolerance — consult your IACUC for monitoring metrics
• 必要时进行干预

其他研究人员具有以下建议

If intake seems to be a problem:

• Determine if a lower dose is effective for your model by conducting a tamoxifen dose feeding trial
• 通过将含他肟烯烃的颗粒与常规饲料颗粒混合来逐步适应小鼠
• 弄湿食物，然后放入笼子里面的菜（需要每日更换）
• Feed tamoxifen diet on weekdays, regular diet on weekends
• 交替的周（Tamoxifen饮食两周，一周休息）持续更长的治疗

安全处理of tamoxifen diets

• Tamoxifen是一种选择性雌激素受体调节剂（SERM），其意味着它可以抑制雌激素的作用或具有促进雌激素效应
• Usual dose for therapeutic effects in humans is 20 mg/day. Tamoxifen diets for rodents typically contain ~one to two mg/pellet
• Accidental tamoxifen exposure can be minimized by using typical lab precautions of lab coat, gloves, and mask when handling the diet.
• 您应联系您的化学安全部门，以便其他机构处理和处理含饮食的饮食的具体指导方针。

Key points from literature

• 治疗长度从一到两周内变化^{1,2,5,6,7,8.8.}到一到两个月^3.,4,8
• Pure tamoxifen1,5,8 and tamoxifen citrate^1,2,3,4,6,7.既有效
  Usual tamoxifen doses are ~40-80 mg per kg body weight per day
• 纯三氧化锡的典型包容为250mg8或500mg1,5，用于柠檬酸三氧肟柠檬酸盐为每千克饮食400毫克^1,2,3,4,6,7.
• Initial weight loss of ten % is reported^1,2,4,7,8.，与减少食物摄入相关²
• Subsequent recovery of body weight after returning to regular diet may be compromised by gene inactivation^2,7

Selected tamoxifen references

¹安德森KB，Winer LH，Mork HK，Molkenterin JD，Jaisser F. 2010。他莫昔芬给药途径和剂量在小鼠心中诱导克雷介导的基因破坏。Transgenic Res 19：715-725。

²蒋晚会，凌俊，济烨，价格DL，AJA SM，WONG PC。2010年。缺失TDP-43下调TBC1D1，一种与肥胖有关的基因，并改变体脂代谢。Proc Natl Acad Sci USA 107:16320-16324。

^3.Kardakaris R，Gareus R，Xanthoulea S，Pasparakis M. 2011。Endothelial and macrophage-specific deficiency of P38alpha MAPK does not affect the pathogenesis of atherosclerosis in ApoE-/- mice.PLoS One 6:e21055。

^4.Kiermayer C, Conrad M, Schneider M, Schmidt J, Brielmeier M. 2007.Optimization of spatiotemporal gene inactivation in mouse heart by oral application of tamoxifen citrate.Genesis 45:11-16。

^5.Koitabashi N，Bedja D，Zaiman Al，Pinto Ym，Zhang M，Gabrielson KL，Takimoto E，Kass Da。2009年。Avoidance of transient cardiomyopathy in cardiomyocyte-targeted tamoxifen-induced MerCreMer gene deletion models.电路res 105：12-15。

^6.Kratsios P C Catela Salimova E,Huth M, Berno V, Rosenthal N, Mourkioti F. 2009.Distinct roles for cell-autonomous Notch signaling in cardiomyocytes of the embryonic and adult heart.PCirc Res 106:559-572。

^7.Miro-Murillo M, Elorza A, Soro-Arnaiz I, Albacete-Albacete L, Ordonez A, Balsa E, Vara-Vega A, Vazquez S, Fuertes E, Fernandez-Criado C, Landazuri MO, Aragones J. 2011.Acute Vhl gene inactivation induces cardiac HIF-dependent erythropoietin gene expression.PLO一6：E22589。

^8.Welle S, Burgess K, Thornton CA, Tawil R. 2009.成熟小鼠肌肉抑制率枯竭与肌肉生长的关系。am j physocrinol metab.oct; 297（4）：e935-40。